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Flowjo price list6/22/2023 Īntibody titration is also an important optimization technique for multiparameter flow cytometry and is the best way to minimize nonspecific binding and increase signal detection. Because this method does not ensure that the brightest signals do not exceed the upper limit of the detector’s range, alternative methods have been developed in which both unstained and brightly stained beads or cells are used to determine MVR. Decreasing the voltage for a detector below its MVR can result in the loss of resolution of dim populations, and increasing the voltage above its MVR gives no advantage for population resolution. In this method, dimly fluorescent beads are run using a series of increasing voltage settings, and the spread of the signal (or the coefficient of variation, CV) is plotted against the voltages. Typically, the voltage walk method ( Figure 1) is used to determine the minimum voltage requirement (MVR) that allows clear resolution of dim fluorescent signals from the background noise of the instrument. For each detector, the voltage (or gain) chosen must provide the best separation between positive and negative signals and ensure all measurements are within the detector’s linear range. Detector optimization takes this process a step further, enabling the highest-quality data to be obtained in each flow cytometer channel. Cytometer manufacturers provide a performance test that certifies the instrument is performing optimally with respect to a precise set of specifications.
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